Staining:Article Title: In-frame deletion in canine PITRM1 is associated with a severe early-onset epilepsy, mitochondrial dysfunction and neurodegeneration
Article Snippet: .. Ultrathin sections were mounted on copper grids, stained with Reynolds lead citrate, and viewed with a Jeol JEM-1400 (Jeol Ltd., Tokyo, Japan) electron microscope equipped with a Gatan Orius SC 1000B bottom mounted CCD-camera (Gatan Inc., USA) at 80 kV. .. Immunohistochemical staining (IHC) with primary antibodies directed against Aβ (mouse monoclonal, 17–24 Antibody, Biolegend), PITRM1 (1:500; PAK0154, rabbit polyclonal; LINARIS Biologische Produkte GmbH, Dossenheim, Germany), VDAC1/Porin (rabbit polyclonal, ab15895, Abcam) and Caspase-3 (rabbit polyclonal, LS-C148242, LSBio), glial fibrillary acidic protein (GFAP, mouse monoclonal, MCA1909 Bio-Rad), Ubiquitin (rabbit polyclonal, ab7780, Abcam), Microtubule-associated-light chain 3B (LC3, rabbit polyclonal, ab48394, Abcam), NADH ubiquinone oxidoreductase (Complex I) subunit NDUF A9 (1:50; MS 111, mouse monoclonal; MitoSciences, Eugene, USA); succinate dehydrogenase complex (Complex II) (1:100; MAK6009, mouse monoclonal; LINARIS Biologische Produkte GmbH, Dossenheim, Germany) ubiquinol cytochrome c oxidoreductase (Complex III) subunit Core 2 (1:250; MS 304, mouse monoclonal; MitoSciences, Eugene, USA), cytochrome c oxidoreductase (Complex IV) subunit I (1:500; MS 404, mouse monoclonal; MitoSciences, Eugene, USA), ATP synthase (Complex V) subunit OSCP (1:250; MS 505, mouse monoclonal; MitoSciences, Eugene, USA) was undertaken.
Article Title: In-frame deletion in canine PITRM1 is associated with a severe early-onset epilepsy, mitochondrial dysfunction and neurodegeneration
Article Snippet: .. Ultrathin sections were mounted on copper grids, stained with Reynolds lead citrate, and viewed with a Jeol JEM-1400 (Jeol Ltd., Tokyo, Japan) electron microscope equipped with a Gatan Orius SC 1000B bottom mounted CCD-camera (Gatan Inc., USA) at 80 kV. .. Immunohistochemical staining (IHC) with primary antibodies directed against Aβ (mouse monoclonal, 17-24 Antibody, Biolegend), PITRM1 (1:500; PAK0154, rabbit polyclonal; LINARIS Biologische Produkte GmbH, Dossenheim, Germany), VDAC1/Porin (rabbit polyclonal, ab15895, Abcam) and Caspase-3 (rabbit polyclonal, LS-C148242, LSBio), glial brillary acidic protein (GFAP, mouse monoclonal, MCA1909 BioRad), Ubiquitin (rabbit polyclonal, ab7780, Abcam), Microtubule-associated-light chain 3B (LC3, rabbit polyclonal, ab48394, Abcam), NADH ubiquinone oxidoreductase (Complex I) subunit NDUF A9 (1:50; MS 111, mouse monoclonal; MitoSciences, Eugene, USA); succinate dehydrogenase complex (Complex II) (1:100; MAK6009, mouse monoclonal; LINARIS Biologische Produkte GmbH, Dossenheim, Germany) ubiquinol cytochrome c oxidoreductase (Complex III) subunit Core 2 (1:250; MS 304, mouse monoclonal; MitoSciences, Eugene, USA), cytochrome c oxidoreductase (Complex IV) subunit I (1:500; MS 404, mouse monoclonal; MitoSciences, Eugene, USA), ATP synthase (Complex V) subunit OSCP (1:250; MS 505, mouse monoclonal; MitoSciences, Eugene, USA) was undertaken.
Microscopy:Article Title: In-frame deletion in canine PITRM1 is associated with a severe early-onset epilepsy, mitochondrial dysfunction and neurodegeneration
Article Snippet: .. Ultrathin sections were mounted on copper grids, stained with Reynolds lead citrate, and viewed with a Jeol JEM-1400 (Jeol Ltd., Tokyo, Japan) electron microscope equipped with a Gatan Orius SC 1000B bottom mounted CCD-camera (Gatan Inc., USA) at 80 kV. .. Immunohistochemical staining (IHC) with primary antibodies directed against Aβ (mouse monoclonal, 17–24 Antibody, Biolegend), PITRM1 (1:500; PAK0154, rabbit polyclonal; LINARIS Biologische Produkte GmbH, Dossenheim, Germany), VDAC1/Porin (rabbit polyclonal, ab15895, Abcam) and Caspase-3 (rabbit polyclonal, LS-C148242, LSBio), glial fibrillary acidic protein (GFAP, mouse monoclonal, MCA1909 Bio-Rad), Ubiquitin (rabbit polyclonal, ab7780, Abcam), Microtubule-associated-light chain 3B (LC3, rabbit polyclonal, ab48394, Abcam), NADH ubiquinone oxidoreductase (Complex I) subunit NDUF A9 (1:50; MS 111, mouse monoclonal; MitoSciences, Eugene, USA); succinate dehydrogenase complex (Complex II) (1:100; MAK6009, mouse monoclonal; LINARIS Biologische Produkte GmbH, Dossenheim, Germany) ubiquinol cytochrome c oxidoreductase (Complex III) subunit Core 2 (1:250; MS 304, mouse monoclonal; MitoSciences, Eugene, USA), cytochrome c oxidoreductase (Complex IV) subunit I (1:500; MS 404, mouse monoclonal; MitoSciences, Eugene, USA), ATP synthase (Complex V) subunit OSCP (1:250; MS 505, mouse monoclonal; MitoSciences, Eugene, USA) was undertaken.
Article Title: Sphingosine kinase 1 overexpression induces MFN2 fragmentation and alters mitochondrial matrix Ca 2+ handling in HeLa cells.
Article Snippet: .. Ultrathin sections were post-stained with uranyl acetate and lead citrate and 30 cell profiles from each culture were systematically sampled and photographed at 6000X with a Jeol JEM-1400 electron microscope equipped with a Gatan Orius SC 1000B bottom mounted CCD-camera. ..
Article Title: OSBP-related protein-2 (ORP2): a novel Akt effector that controls cellular energy metabolism.
Article Snippet: .. Specimens were observed using Jeol JEM 1400 (Jeol Ltd., Tokyo, Japan) microscope equipped with Orius SC 1000B bottom mounted CCD camera (Gatan Inc., USA) at an acceleration voltage of 80 kV. ..
Article Title: Genome-wide RNAi screen for nuclear actin reveals a network of cofilin regulators
Article Snippet: .. Sections (90-nm thick) were cut parallel to the coverslip, post-stained with uranyl acetate and lead citrate, and imaged with a Jeol JEM-1400 (Jeol Ltd., Tokyo, Japan) microscope, equipped with a Gatan Orius SC 1000B bottom mounted CCD-camera (Gatan Inc., USA), operating at 80 kV. ..
Article Title: In-frame deletion in canine PITRM1 is associated with a severe early-onset epilepsy, mitochondrial dysfunction and neurodegeneration
Article Snippet: .. Ultrathin sections were mounted on copper grids, stained with Reynolds lead citrate, and viewed with a Jeol JEM-1400 (Jeol Ltd., Tokyo, Japan) electron microscope equipped with a Gatan Orius SC 1000B bottom mounted CCD-camera (Gatan Inc., USA) at 80 kV. .. Immunohistochemical staining (IHC) with primary antibodies directed against Aβ (mouse monoclonal, 17-24 Antibody, Biolegend), PITRM1 (1:500; PAK0154, rabbit polyclonal; LINARIS Biologische Produkte GmbH, Dossenheim, Germany), VDAC1/Porin (rabbit polyclonal, ab15895, Abcam) and Caspase-3 (rabbit polyclonal, LS-C148242, LSBio), glial brillary acidic protein (GFAP, mouse monoclonal, MCA1909 BioRad), Ubiquitin (rabbit polyclonal, ab7780, Abcam), Microtubule-associated-light chain 3B (LC3, rabbit polyclonal, ab48394, Abcam), NADH ubiquinone oxidoreductase (Complex I) subunit NDUF A9 (1:50; MS 111, mouse monoclonal; MitoSciences, Eugene, USA); succinate dehydrogenase complex (Complex II) (1:100; MAK6009, mouse monoclonal; LINARIS Biologische Produkte GmbH, Dossenheim, Germany) ubiquinol cytochrome c oxidoreductase (Complex III) subunit Core 2 (1:250; MS 304, mouse monoclonal; MitoSciences, Eugene, USA), cytochrome c oxidoreductase (Complex IV) subunit I (1:500; MS 404, mouse monoclonal; MitoSciences, Eugene, USA), ATP synthase (Complex V) subunit OSCP (1:250; MS 505, mouse monoclonal; MitoSciences, Eugene, USA) was undertaken.
Imaging:Article Title: Versatile Trans -Replication Systems for Chikungunya Virus Allow Functional Analysis and Tagging of Every Replicase Protein
Article Snippet: Positive cells, identified during fluorescent and DIC imaging, were located for each sample using Jeol JEM-1400 transmission electron microscope. .. Gatan Orius SC 1000B bottom mounted CCD-camera was used for imaging. ..
Transmission Electron Microscopy:Article Title: REEP3 and REEP4 determine the tubular morphology of the endoplasmic reticulum during mitosis
Article Snippet: .. TEM images were acquired using Jeol JEM-1400 (Jeol Ltd., Tokyo, Japan) operating at 80kV equipped with Gatan Orius SC 1000B bottom mounted CCD-camera (Gatan Inc., USA). ..
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